PCBP2促进口蹄疫病毒增殖的机制研究

doi:10.11843/j.issn.0366-6964.2018.07.014

Abstract

Abstract:

Poly(rC) binding protein 2(PCBP2) belongs to a class of proteins that bind to poly(C) stretches of both RNA and DNA. PCBP2 has roles in maintaining mRNA stability and regulating translation. Meanwhile PCBP2 is a negative regulator in VISA-mediated antiviral signaling to reduce production of IFN-β. Previous study have shown that PCBP2 can affect the multiplication of foot-and-mouth disease virus(FMDV), but the specific mechanism is unclear. Here, we investigated the molecular mechanisms about PCBP2 promoting the multiplication of FMDV. Results:1) We found that FMDV structural protein VP0, 2B, VP2, 2C and 3D can interact with PCBP2 via Co-IP and GST pull-down tests. 2) Further study revealed that PCBP2 is a negative regulator in VISA-mediated antiviral signaling, and 2C, 3D, VP0 and 2B can promote the negative regulation of PCBP2 on VISA-mediated antiviral signaling via the Dual-Luciferase Reporter Assay. 3) Meanwhile we found that FMDV VP0 can increase specific degradation of VISA mediated by PCBP2 through Western blot. In a word, FMDV VP0 can increase degradation of VISA mediated by PCBP2 via VP0-PCBP2 interaction to reduce the production of IFN-β, and enhance the FMDV activity in cell.

CLC Number:

S852.659.6

HE Yan-chun, YANG Wen-ping, FU Shao-zu, ZHENG Hai-xue, YANG Xiao-pu. The Research on Molecular Mechanism of PCBP2 Promoting the Multiplication of Foot-and-mouth Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1451-1459.

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The Research on Molecular Mechanism of PCBP2 Promoting the Multiplication of Foot-and-mouth Disease Virus

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